Poultry Diagnostic Laboratory
RD 6, Box 48
Georgetown, DE 19947
Salem, M.V.Z., M.S.
Senior Scientist and Poultry Pathologist
Ziegler, D.V.M, M.P.V.M
Associate Scientist and Poultry Pathologist
Poultry Diagnostic Laboratory Submissions
Types of avian cases received
Cornish, Broilers, Roasters, Broiler Breeders, Breeder Replacements, Layers
Miscellaneous cases submitted through lab and processed elsewhere. Feed samples for toxicosis: Drug, agricultural chemicals, heavy metals, mycotoxins.
Laboratory Services Offered
Microbiology - Cultures for isolation and identification. Antibiotic sensitivity testing performed
Bacterial organisms frequently isolated
|E. Coli||Pastuerella sp.|
|Staph sp.||Salmonella sp.|
|Clostridia sp.||Mycoplasma sp.|
Cases suspected to be of viral etiology or association are sampled for virus isolation. Samples are maintained in frozen tryptose phosphate broth an transported to the Animal Science lab in Newark for virus isolation attempts.
Sources for collection of virus isolation specimens: Cloaca, gut, hock joint, lungs, spleen, trachea, tendon, bursa, brain.
Viruses commonly isolated from poultry:
|Newcastle Disease, NDV||Reovirus|
|Infectious Bronchitis, IBV||Adenovirus|
|Infectious Laryngotracheitis, ILTV||Infectious Bursal Disease (Gumboro) IBD|
Mycology - Molds, fungi isolated
Mold plate counts performed
Agar gel precipitin immunodiffusion tests performed
IBD - infectious bursal disease
AI - avian influenza
Hemagglutination inhibition tests
NDV - Newcastle disease virus
MG, MS - Mycoplasma gallisepticum and Mycoplasma synoviae
Rapid Plate agglutination tests
ELISA (Enzyme-linked Immunosorbent Assay) - quantitatively measures antibody levels. Attributes: Specificity, sensitivity, speeds of assay, and low cost.
Processing plant information
Breeder flock monitoring
Coccidiosis (Eimera mitis/mivati, E. acervulina, E. maxima, E. necatrix, E. tenella)
Capillarial worms (capillaria sp.)
Cecal worms (Heterakis gallinae)
Roundworms (Ascardia galli)
Tapeworms (Rallentina cesticillus)
Fluorescent Antibody Procedures
MG, MM, MS - Mycoplasma gallisepticum, M. Meleagridis, M. Synoviae
Histopathology - (Conducted in Animal Science lab, Newark by Dr. Conrad Pope)
Histology - (micro-anatomy)
Histopathology - (micro-pathology) - Determination of microscopic pathological lesions. Must be correlated to clinical disease and gross pathology for maximum effectiveness. Most lesions are not disease specific but must be interpreted considering all available information. Specimens collected and transferred to laboratory for processing and evaluation by out histopathologist.
Designed to evaluate the efficacy of vaccines for research and the commercial poultry industry.
Determine the resistance of susceptibility of chickens to disease agent.
Challenges conducted in colony house and Horsfal isolation units are located in a fence in, secured area away from the diagnostic lab. Horsfal isolation units are located in a remote entry unit of the laboratory.